Specimen | MHP DNA detection | Room | |||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | ||
1 MHP-inoculated pig: 8 uninoculated pigs | 3 MHP-inoculated pigs: 6 uninoculated pigs | 6 MHP-inoculated pigs: 3 uninoculated pigs | 9 MHP-inoculated pigs | ||
Tracheal swab | First detection | DPI 3 | DPI 3 | DPI 3 | DPI 3 |
 | Pig-to-pig transmission detecteda | DPI 21 | DPI 10 | DPI 7 | NAb |
 | 100% detection in group | DPI 52 | DPI 38 | DPI 21 | DPI 7 |
 | MHP PCR positive of total samples | 47 of 99 | 69 of 107 | 94 of 104 | 98 of 103 |
 | MHP PCR overall mean ± se Ctc | 26.8 ± 0.7 | 27.6 ± 0.6 | 26.2 ± 0.4 | 26.2 ± 0.4 |
 | Highest MHP DNA concentration (DPI) | 23.4 (DPI 59) | 24.4 (DPI 45) | 23.6 (DPI 28) | 24.3 (DPI 28) |
Oral fluid | First detection | DPI 8 | DPI 9 | DPI 14 | DPI 8 |
 | MHP PCR positive of total samplesd | 17 of 591 | 32 of 592 | 43 of 592 | 31 of 592 |
 | MHP PCR overall mean ± se Ct | 34.7 ± 0.3 | 33.3 ± 0.5 | 33.5 ± 0.3 | 33.4 ± 0.4 |
 | MHP PCR lowest Ct | 31.6 (DPI 51) | 25.7 (DPI 13) | 27.4 (DPI 45) | 26.1 (DPI 28) |
Drinker | First detection | DPI 21 | DPI 21 | DPI 21 | DPI 21 |
 | MHP PCR positive of total samples | 5 of 8 | 4 of 8 | 4 of 8 | 5 of 8 |
 | MHP PCR overall mean ± se Ct | 35.3 ± 1.5 | 33.7 ± 1.9 | 34.7 ± 1.4 | 34.7 ± 1.6 |
 | MHP PCR lowest Ct | 34.5 (DPI 59) | 31.5 (DPI 21) | 33.3 (DPI 35) | 32.2 (DPI 24) |
Air | First detection | NDe | DPI 10 | NDe | DPI 17 |
 | MHP PCR positive of total samples | NDe | 5 of 26 | NDe | 3 of 26 |
 | MHP PCR overall mean ± se Ct |  | 36.2 ± 0.4 |  | 36.3 ± 0.6 |