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Fig. 4 | Porcine Health Management

Fig. 4

From: Emergence of a virulent porcine reproductive and respiratory syndrome virus (PRRSV) 1 strain in Lower Austria

Fig. 4

Virus isolation of field strain AUT15-33. Serum of nine acutely affected nursery piglets was inoculated on porcine alveolar macrophages (PAM). After two days cells were fixed with methanol-acetone and immunofluorescence stained with an anti-PRRSV-N-protein monoclonal antibody (kindly provided by A. Saalmüller, Vienna) [39] and goat-anti-mouse conjugated with Cy3 as a secondary antibody. Varying cytopathic effects were seen and a PRRSV-specific immunofluorescence staining was detected (serum samples #33 and 38 are shown exemplarily)

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Porcine Health Management

ISSN: 2055-5660

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