| |
qRT-PCR mean log RNA copies/mL (± SD)
|
|---|
|
Days
|
37 °C
|
45 °C
|
50 °C
|
55 °C
|
60 °C
|
65 °C
|
70 °C
|
|---|
|
0
|
8.57 (0.02)
|
8.43 (0.02)
|
8.40 (0.08)
|
8.44 (0.03)
|
8.41 (0.08)
|
8.41 (0.11)
|
8.57 (0.36)
|
|
1
|
8.45 (0.05)
|
7.53 (0.04)
|
6.58 (0.32)
|
5.53 (0.15)
|
4.69 (0.11)
|
4.58 (0.51)
|
4.67 (0.12)
|
|
2
|
8.26 (0.03)
|
6.27 (0.42)
|
4.67 (0.10)
|
4.85 (0.25)
|
4.83 (0.02)
|
4.41 (0.60)
|
4.35 (0.12)
|
|
3
|
7.85 (0.09)
|
5.25 (0.08)
|
4.91 (0.06)
|
4.85 (0.13)
|
5.03 (0.08)
|
4.37 (0.08)
|
3.81 (0.40)
|
|
4
|
7.64 (0.19)
|
4.43 (0.11)
|
4.56 (0.19)
|
4.89 (0.07)
|
5.16 (0.06)
|
4.65 (0.32)
|
3.79 (0.48)
|
|
5
|
7.48 (0.11)
|
4.43 (0.17)
|
4.69 (0.11)
|
4.79 (0.31)
|
4.60 (0.06)
|
4.34 (0.05)
|
3.58 (0.09)
|
|
7
|
6.02 (0.06)
|
5.16 (0.75)
|
5.27 (0.19)
|
4.69 (0.82)
|
5.09 (0.28)
|
3.91 (0.18)
|
3.13 (0.07)
|
|
14
|
4.55 (0.22)
|
4.43 (0.50)
|
5.21 (0.05)
|
4.83 (0.05)
|
4.41 (0.09)
|
3.16 (0.65)
|
0.78 (0.13)a
|
-
a2 of 3 samples negative (below detection limit of 8.8 RNA copies per reaction)
