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Table 1 Summary of time-temperature trial qRT-PCR assay results as RNA copy equivalents

From: Effectiveness of composting as a biosecure disposal method for porcine epidemic diarrhea virus (PEDV)-infected pig carcasses

  qRT-PCR mean log RNA copies/mL (± SD)
Days 37 °C 45 °C 50 °C 55 °C 60 °C 65 °C 70 °C
0 8.57 (0.02) 8.43 (0.02) 8.40 (0.08) 8.44 (0.03) 8.41 (0.08) 8.41 (0.11) 8.57 (0.36)
1 8.45 (0.05) 7.53 (0.04) 6.58 (0.32) 5.53 (0.15) 4.69 (0.11) 4.58 (0.51) 4.67 (0.12)
2 8.26 (0.03) 6.27 (0.42) 4.67 (0.10) 4.85 (0.25) 4.83 (0.02) 4.41 (0.60) 4.35 (0.12)
3 7.85 (0.09) 5.25 (0.08) 4.91 (0.06) 4.85 (0.13) 5.03 (0.08) 4.37 (0.08) 3.81 (0.40)
4 7.64 (0.19) 4.43 (0.11) 4.56 (0.19) 4.89 (0.07) 5.16 (0.06) 4.65 (0.32) 3.79 (0.48)
5 7.48 (0.11) 4.43 (0.17) 4.69 (0.11) 4.79 (0.31) 4.60 (0.06) 4.34 (0.05) 3.58 (0.09)
7 6.02 (0.06) 5.16 (0.75) 5.27 (0.19) 4.69 (0.82) 5.09 (0.28) 3.91 (0.18) 3.13 (0.07)
14 4.55 (0.22) 4.43 (0.50) 5.21 (0.05) 4.83 (0.05) 4.41 (0.09) 3.16 (0.65) 0.78 (0.13)a
  1. a2 of 3 samples negative (below detection limit of 8.8 RNA copies per reaction)