Table 1 Summary of time-temperature trial qRT-PCR assay results as RNA copy equivalents
qRT-PCR mean log RNA copies/mL (± SD) | |||||||
|---|---|---|---|---|---|---|---|
Days | 37 °C | 45 °C | 50 °C | 55 °C | 60 °C | 65 °C | 70 °C |
0 | 8.57 (0.02) | 8.43 (0.02) | 8.40 (0.08) | 8.44 (0.03) | 8.41 (0.08) | 8.41 (0.11) | 8.57 (0.36) |
1 | 8.45 (0.05) | 7.53 (0.04) | 6.58 (0.32) | 5.53 (0.15) | 4.69 (0.11) | 4.58 (0.51) | 4.67 (0.12) |
2 | 8.26 (0.03) | 6.27 (0.42) | 4.67 (0.10) | 4.85 (0.25) | 4.83 (0.02) | 4.41 (0.60) | 4.35 (0.12) |
3 | 7.85 (0.09) | 5.25 (0.08) | 4.91 (0.06) | 4.85 (0.13) | 5.03 (0.08) | 4.37 (0.08) | 3.81 (0.40) |
4 | 7.64 (0.19) | 4.43 (0.11) | 4.56 (0.19) | 4.89 (0.07) | 5.16 (0.06) | 4.65 (0.32) | 3.79 (0.48) |
5 | 7.48 (0.11) | 4.43 (0.17) | 4.69 (0.11) | 4.79 (0.31) | 4.60 (0.06) | 4.34 (0.05) | 3.58 (0.09) |
7 | 6.02 (0.06) | 5.16 (0.75) | 5.27 (0.19) | 4.69 (0.82) | 5.09 (0.28) | 3.91 (0.18) | 3.13 (0.07) |
14 | 4.55 (0.22) | 4.43 (0.50) | 5.21 (0.05) | 4.83 (0.05) | 4.41 (0.09) | 3.16 (0.65) | 0.78 (0.13)a |