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Table 1 Mycoplasma hyopneumoniae standard ELISA (Idexx HerdChek Mhyo ELISA, indirect ELISA; Idexx Laboratories; S/P-ratio, sample to positive ratio) monitoring results from March 2017 onwards. Results of the second ELISA test (IDEIA™ Mycoplasma hyopneumoniae; Oxoid – Thermo Scientific; PI, percentage of inhibition) are also given. Table demonstrates total number of samples and number of samples with negative (Idexx, S/P < 0.30; IDEIA™, PI ≥ 65%), suspect (Idexx, 0.30 ≤ S/P ≤ 0.40; IDEIA™, 50% ≥ PI > 65%) or positive (Idexx, S/P > 0.40; IDEIA™, PI < 50%) M. hyopneumoniae ELISA results. Mean titers (± SEM) for Idexx ELISA (expressed as S/P-ratio) and IDEIA™ ELISA (expressed as PI) are given per sampling timepoint

From: Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd

Date Analytical test Analytical result Results (mean ± SEM) Total sample number
Negative Suspect Positive  
20.3.2017 Idexx 0 1 9 0.86 ± 0.15 10
IDEIA™ 2 7 1 59.7 ± 7.7% 10
21.4.2017 Idexx 11 7 7 0.43 ± 0.08 25
IDEIA™ 17 4 4 65.4 ± 14.3% 25
25.4.2017 Idexx 18 0 4 0.17 ± 0.04 22
IDEIA™ 4 0 0 90.3 ± 15.0% 4
9.8.2017 Idexx 11 1 8 0.36 ± 0.06 20
IDEIA™ 2 0 2 58.0 ± 11.8% 4