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Table 1 Mycoplasma hyopneumoniae standard ELISA (Idexx HerdChek Mhyo ELISA, indirect ELISA; Idexx Laboratories; S/P-ratio, sample to positive ratio) monitoring results from March 2017 onwards. Results of the second ELISA test (IDEIA™ Mycoplasma hyopneumoniae; Oxoid – Thermo Scientific; PI, percentage of inhibition) are also given. Table demonstrates total number of samples and number of samples with negative (Idexx, S/P < 0.30; IDEIA™, PI ≥ 65%), suspect (Idexx, 0.30 ≤ S/P ≤ 0.40; IDEIA™, 50% ≥ PI > 65%) or positive (Idexx, S/P > 0.40; IDEIA™, PI < 50%) M. hyopneumoniae ELISA results. Mean titers (± SEM) for Idexx ELISA (expressed as S/P-ratio) and IDEIA™ ELISA (expressed as PI) are given per sampling timepoint

From: Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd

Date

Analytical test

Analytical result

Results (mean ± SEM)

Total sample number

Negative

Suspect

Positive

 

20.3.2017

Idexx

0

1

9

0.86 ± 0.15

10

IDEIA™

2

7

1

59.7 ± 7.7%

10

21.4.2017

Idexx

11

7

7

0.43 ± 0.08

25

IDEIA™

17

4

4

65.4 ± 14.3%

25

25.4.2017

Idexx

18

0

4

0.17 ± 0.04

22

IDEIA™

4

0

0

90.3 ± 15.0%

4

9.8.2017

Idexx

11

1

8

0.36 ± 0.06

20

IDEIA™

2

0

2

58.0 ± 11.8%

4