Porcine Health Management

Table 4 In vitro fertilization parameters of fresh and vitrified oocytes

From: An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes

Treatment	Total Oocyte n	Pronuclear formation (%)
Treatment	Total Oocyte n	1PN	2PN + 2 PBs	>2PN	Total Fertilization	Non-fertilized
IVF
Control (DO-GC)	41	10 (24 ± 1.2)^a	21 (51 ± 3.4)^a	8 (20 ± 5)^a	29 (71 ± 2)^a	2 (5 ± 1.3)^a
Vitrification	62	16 (26 ± 3.2)^a	38 (61 ± 4.3)^a	8 (13 ± 2.8)^a	46 (75 ± 3.2)^a	–
ICSI
Control (DO-GC)	30	5 (17 ± 8)^a	10 (33 ± 4.2)^b	.	10 (33 ± 4.3)^b	15 (50 ± 1.1)^b
Vitrification	43	3 (7 ± 1.9)^b	15 (35 ± 8)^b	–	15 (35 ± 8)^b	25 (58 ± 1.2)^b
PICSI
Control (DO-GC)	45	10 (22 ± 4)^a	22 (49 ± 9.9)^a	–	22 (49 ± 9.9)^c	13 (29 ± 6.2)^c
Vitrification	40	8 (20 ± 3)^a	18 (45 ± 5.6)^c	–	18 (45 ± 5.6)^c	14 (35 ± 1.4)^c

DO-GC Denuded oocytes cultured with granulosa cells, PBs polar bodies, PN pronucleus,
Total Fertilization = counted as 2PN + >2PN/total oocytes
Non-fertilized = non pronuclear formation/total oocytes
Percentage data are presented as mean ± SD values
^a,b,cValues in the same column with different letters are significantly different (P < 0.05)

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ISSN: 2055-5660

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