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Table 4 In vitro fertilization parameters of fresh and vitrified oocytes

From: An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes

Treatment Total Oocyte
n
Pronuclear formation (%)
1PN 2PN + 2 PBs >2PN Total Fertilization Non-fertilized
IVF
 Control (DO-GC) 41 10 (24 ± 1.2)a 21 (51 ± 3.4)a 8 (20 ± 5)a 29 (71 ± 2)a 2 (5 ± 1.3)a
 Vitrification 62 16 (26 ± 3.2)a 38 (61 ± 4.3)a 8 (13 ± 2.8)a 46 (75 ± 3.2)a
ICSI
 Control (DO-GC) 30 5 (17 ± 8)a 10 (33 ± 4.2)b . 10 (33 ± 4.3)b 15 (50 ± 1.1)b
 Vitrification 43 3 (7 ± 1.9)b 15 (35 ± 8)b 15 (35 ± 8)b 25 (58 ± 1.2)b
PICSI
 Control (DO-GC) 45 10 (22 ± 4)a 22 (49 ± 9.9)a 22 (49 ± 9.9)c 13 (29 ± 6.2)c
 Vitrification 40 8 (20 ± 3)a 18 (45 ± 5.6)c 18 (45 ± 5.6)c 14 (35 ± 1.4)c
  1. DO-GC Denuded oocytes cultured with granulosa cells, PBs polar bodies, PN pronucleus,
  2. Total Fertilization = counted as 2PN + >2PN/total oocytes
  3. Non-fertilized = non pronuclear formation/total oocytes
  4. Percentage data are presented as mean ± SD values
  5. a,b,cValues in the same column with different letters are significantly different (P < 0.05)