Porcine Health Management

Table 5 In vitro embryo development and blastocyst quality of fresh and vitrified oocytes

From: An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes

Treatment	Total n	Cleavage (%)	Blastocyst (%)	Viable cells in blastocysts %	Total no. of nuclei (means ± S.D.)
IVF
Control (DO-GC)	100	73 (73 ± 3.4)^a	15 (15 ± 1.2)^a	82 ± 3^a	50 ± 0.6^a
Vitrification	210	142 (68 ± 2)^a	30 (14 ± 1.8)^a	70 ± 5^b	46 ± 3^a
ICSI
Control (DO-GC)	74	33 (45 ± 2)^b	9 (12 ± 0.3)^a	100^c	44 ± 0.9^b
Vitrification	113	45 (40 ± 9)^b	10 (9 ± 0.2)^b	42 ± 5^d	41 ± 2^b
PICSI
Control (DO-GC)	60	38 (63 ± 2)^a	18 (30 ± 1.5)^c	100^c	50 ± 2.5^a
Vitrification	158	99 (63 ± 3.5)^a	39 (25 ± 3)^c	100^c	54 ± 5^a

DO-GC Denuded oocytes cultured with granulosa cells, n number of embryos examined
Cell viability was considered as the percentage of viable cells in blastocysts
Percentage data are presented as mean ± SD values
Cleavage = number of zygotes cleaved per total cultivated
Blastocyst = number of blastocysts per total cultivated
^a,b,c,dValues in the same column with different letters are significantly different (P < 0.05)

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ISSN: 2055-5660

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