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Table 3 Cytokine measure geometric mean (±SD) results

From: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine

  Treatment Day of study
3 7 28 83 125 (DC-1) 132 (DC + 6)
IL-10 T01 ND ND     4.8 ± 1.0 (1/5)
T02 ND ND     5.2 ± 1.7 (1/8)
T03 ND ND     10.1 ± 6.4 (2/8)
P value T01 vs T02       NS
T01 vs T03       NS
T02 vs T03       NS
IL-8 T01 ND ND     48.1 ± 22.9 (3/5)
T02 ND ND     33.8 ± 12.7 (3/8)
T03 ND ND     69.6 ± 26.2 (6/8)
P value T01 vs T02       NS
T01 vs T03       NS
T02 vs T03       NS
IFN-α T01 ND 1.9 ± 0.0 (1/5)     
T02 9.8 ± 6.1 (10/10) 32.8 ± 11.8 (10/10)     
T03 9.5 ± 9.7 (9/10) 14.1 ± 9.9 (10/10)     
TNF-α T01 ND ND (1/5)a ND 62.7 ± 7.6 (1/5) ND 29.1 ± 1.5
T02 ND ND ND ND (1/8)a ND 41.9 ± 1.6 (1/8)
T03 ND ND ND ND (2/8)a ND 78.2 ± 2.9 (1/8)
P value T01 vs T02       NS
T01 vs T03       NS
T02 vs T03       NS
IFN-γ T01    1.6 ± 0.0 (5/5) 1.6 ± 0.0 (4/5) 0.0 ± 0.0 (3/4) 8.1 ± 0.5 (3/4)
T02    22.1 ± 1.1 (9/10) 80.5 ± 8.2 (8/8) 166.5 ± 7.1 (8/8) 133.5 ± 5.7 (8/8)
T03    51.3 ± 4.1 (8/9) 77.2 ± 8.2 (8/8) 102.2 ± 4.3 (8/8) 155.7 ± 6.6 (8/8)
P value T01 vs T02      0.0003 0.0033
T01 vs T03      0.0108 0.0011
T02 vs T03      NS NS
  1. aAlthough some pigs had values above the cut-off of the technique, the mean value was below the cut-off
  2. Results from IL-10, IL-8, IFN-α and TNF-α ELISA tests are expressed as pg/mL of serum. Results from IFN-γ ELISPOT are expressed as number of IFN-γ- secreting cells /5 × 105 PBMC. In brackets, the number of positive animals per treatment and time point. DC day of challenge, ND non-detectable (below limit of detection of the technique), NS non-significant; blank cell indicates not measured