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Table 2 Thermal cycling profile used for one-step RT-PCRs developed in the present study as part of the in-house primer-walking approach to amplify the whole genome sequence of swine NoVs

From: Identification of two divergent swine Noroviruses detected at the slaughterhouse in North East Italy

Thermal cycling profileTemperatureDurationN° cycles
Reverse transcription reaction50 °C60 min 
Polymerase Activation94 °C2 min 
Denaturation94 °C15 sX40
Annealing50~60 °C30 s
Elongation68 °C1~2 min
Final elongation68 °C5 min