Table 2 Thermal cycling profile used for one-step RT-PCRs developed in the present study as part of the in-house primer-walking approach to amplify the whole genome sequence of swine NoVs
From: Identification of two divergent swine Noroviruses detected at the slaughterhouse in North East Italy
Thermal cycling profile | Temperature | Duration | N° cycles |
|---|---|---|---|
Reverse transcription reaction | 50 °C | 60 min |  |
Polymerase Activation | 94 °C | 2 min |  |
Denaturation | 94 °C | 15 s | X40 |
Annealing | 50~60 °C | 30 s | |
Elongation | 68 °C | 1~2 min | |
Final elongation | 68 °C | 5 min |  |