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Table 4 Results of susceptibility testing and multilocus sequence typing (MLST) (PubMLST) of Brachyspira hyodysenteriae isolates obtained before and after the eradication program on the 10 farms. Farms with successful eradication are marked in bold

From: Implementation and evaluation of different eradication strategies for Brachyspira hyodysenteriae

  Farm number
1 2 3 4 5 6 7 8 9 10
Before eradication
 Detected by … PCR PCR, culture PCR, culture culture PCR culture PCR, culture culture culture culture
 Number of isolates 0 1 1 1 0 1 1 1 1 1
 MIC-values (μg/ml) b
  Tiamulin   > 16 0.16 0.12   2 2 0.50 > 16 > 16
  Valnemulin   > 16 8 0.12   4 4 1 > 16 > 16
  Tylvalosin   > 128 0.25 16   8 8 128 > 128 > 128
 MLST typea    ST265     ST228   ST87  
Eradication successful yes yes no yes no no no yes no no
After eradication
 Detected by …    culture   PCR, culture PCR culture   culture PCR
 Number of isolates    1   1 0 1   1 0
 MIC-value (μg/ml) b
  Tiamulin    2   < 0.03   16   > 16  
  Valnemulin    8   < 0.03   4   > 16  
  Tylvalosin    0.12   1   32   128  
 MLST type a    ST265     ST264   ST87  
  1. a MLST analysis was only performed on farms where B. hyodysenteriae isolates could be obtained before and after the eradication program, allowing comparison of MLST types
  2. b Isolates were considered resistant if MIC-values (μg/ml) were higher than 2, 2 and 32 for tiamulin, valnemulin and tylvalosin, respectively [24, 25]