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Table 4 Results of susceptibility testing and multilocus sequence typing (MLST) (PubMLST) of Brachyspira hyodysenteriae isolates obtained before and after the eradication program on the 10 farms. Farms with successful eradication are marked in bold

From: Implementation and evaluation of different eradication strategies for Brachyspira hyodysenteriae

 

Farm number

1

2

3

4

5

6

7

8

9

10

Before eradication

 Detected by …

PCR

PCR, culture

PCR, culture

culture

PCR

culture

PCR, culture

culture

culture

culture

 Number of isolates

0

1

1

1

0

1

1

1

1

1

 MIC-values (μg/ml) b

  Tiamulin

 

> 16

0.16

0.12

 

2

2

0.50

> 16

> 16

  Valnemulin

 

> 16

8

0.12

 

4

4

1

> 16

> 16

  Tylvalosin

 

> 128

0.25

16

 

8

8

128

> 128

> 128

 MLST typea

  

ST265

   

ST228

 

ST87

 

Eradication successful

yes

yes

no

yes

no

no

no

yes

no

no

After eradication

 Detected by …

  

culture

 

PCR, culture

PCR

culture

 

culture

PCR

 Number of isolates

  

1

 

1

0

1

 

1

0

 MIC-value (μg/ml) b

  Tiamulin

  

2

 

< 0.03

 

16

 

> 16

 

  Valnemulin

  

8

 

< 0.03

 

4

 

> 16

 

  Tylvalosin

  

0.12

 

1

 

32

 

128

 

 MLST type a

  

ST265

   

ST264

 

ST87

 
  1. a MLST analysis was only performed on farms where B. hyodysenteriae isolates could be obtained before and after the eradication program, allowing comparison of MLST types
  2. b Isolates were considered resistant if MIC-values (μg/ml) were higher than 2, 2 and 32 for tiamulin, valnemulin and tylvalosin, respectively [24, 25]
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Porcine Health Management

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