Fig. 1

Gating strategy – analysis of leukocyte populations. Doublet events were discriminated on the basis of forward scatter integral versus forward scatter peak height (a). Dead cells were excluded from analysis using propidium iodide staining (b). Leucocytes were determined as CD45⁺ cells (c). Granulocytes were determined as CD45⁺CD172α⁺swc8⁺, macrophages as CD45⁺CD172α⁺swc8⁻ and lymphocytes as CD45⁺CD172α⁻swc8⁻ (d)