Table 2 Primary and secondary reagents used for flow cytometry analysis
Antigen | Clone | Isotype | Flourochrome | Labeling strategy | Source of primary antibody |
|---|---|---|---|---|---|
Leukocyte staining panel | |||||
CD45 | K252 1E4 | IgG1 | Alexa Fluor 647 | Secondary antibodya | A generous gift from Dr. K. Haverson, University of Bristol, UK |
CD172α | 74–22-15 | IgG2b | Alexa Fluor 488 | Secondary antibodyb | WSU Monoclonal Antibody Center |
swc8 | MIL3 | IgM | PE | Secondary antibodyc | A generous gift from Dr. J.K. Lunney, Animal Parasitology Institute, Beltsville, USA |
Lymphocyte subpopulations staining panel | |||||
CD2 | PG168A | IgG3 | Alexa Fluor 488 | Secondary antibodyd | WSU Monoclonal Antibody Center |
CD3 | PPT3 | IgG1 | PE | Zenon labelinge | BD Biosciences |
CD4 | 74–12-4 | IgG2b | DyLight405 | Secondary antibodyf | WSU Monoclonal Antibody Center |
CD8α | 76–2-11 | IgG2a | Alexa Fluor 647 | Secondary antibodyg | WSU Monoclonal Antibody Center |
γδTCR | PGBL22A | IgG1 | PE-TXRD | Secondary antibodyh | WSU Monoclonal Antibody Center |