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Fig. 3 | Porcine Health Management

Fig. 3

From: Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication

Fig. 3

Representative images of GJIC evaluation after COCs vitrification. a Cumulus cell-oocyte GJIC was measured by the quantitative FI profiles of calcein in COCs. Lines were drawn through the COCs optical sections generated by the confocal microscope (red line). In the control group, high FI was observed in the CCs and oocyte at 4 and 8 h (red lines). Reduced FI was observed in carbenoxolone, toxicity, and vitrification groups after 4 and 8 h. Carbenoxolone was used as a positive control of GJIC blockage (red crossed lines). At 22 and 44 h of culture, reduced FI was observed in all groups. b Representative FI profiles in COCs from all groups. Graphs show the FI profiles of calcein (marked in green) transferred from the CCs to the oocyte. GJIC = gap junction intercellular communication; FI = fluorescence intensity. Scale bar = 30 μM

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