Fig. 1From: DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo developmentViability evaluation in oocytes and cumulus cells. Representative images from oocytes (a, b) and cumulus cells (c, d) for viability evaluation in different groups at 40×. Oocyte (n = 304) and cumulus cells (n = 400) viability was evaluated after 44 h of in vitro maturation. For oocytes and cumulus cells, different staining agents were used: methyl-thiazolyl-tetrazolium (MTT) and trypan blue, respectively. a Stained purple oocyte: alive; b unstained oocyte: dead; c unstained cumulus cells: alive; d stained cumulus cells: dead. Scale bar: 30 μm. n = number of evaluated cellsBack to article page