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Fig. 2 | Porcine Health Management

Fig. 2

From: DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development

Fig. 2

Percentage of oocyte and cumulus cells viability. Hydrogen peroxide (COCs exposed to 2.2% H2O2), Toxicity (16% EG + DMSO), and Vitrification (EG + DMSO + Vitrification). Cumulus cells (CCs) were removed from oocytes (n = 304) for evaluation. Control (n = 242 evaluated CCs), H2O2 (n = 263 evaluated CCs), Toxicity (n = 204 evaluated CCs), Vitrification (n = 219 evaluated CCs). In all groups, decreased viability was observed in both cell types compared to control. Compared to oocytes, CCs viability decreased significantly in all groups. Data are presented as mean ± standard deviation (SD). Significant differences were considered when P < 0.0001. *Indicates significant difference versus control. **Indicates significant difference between oocytes and cumulus cells. H2O2 = hydrogen peroxide; EG = ethylene glycol; DMSO = dimethylsulphoxide. n = number of evaluated cells

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Porcine Health Management

ISSN: 2055-5660

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