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Fig. 6 | Porcine Health Management

Fig. 6

From: DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development

Fig. 6

In vitro fertilization, cleavage, and blastocyst rates evaluation. Toxicity (16% EG + DMSO), and Vitrification (EG + DMSO + Vitrification). After treatments, to determine the importance of the cumulus cells during fertilization and embryo development, oocytes were fertilized in the absence (− CCs) or presence (+ CCs) of the CCs. The number of evaluated oocytes/treatment was n = 214. Oocyte fertilization, cleavage, and blastocyst rates increased significantly with the presence (+ CCs) of CCs compared to denuded oocytes in control groups. However, fertilization, cleavage and blastocyst rates significantly decreased in the Toxicity and Vitrification groups. Also, the presence of CCs in the toxicity and vitrification groups did not increase fertilization, cleavage, and blastocyst rates compared to control. Data are presented as mean ± standard deviation (SD). Significant differences were considered when P < 0.0001. *Indicates significant difference versus the respective control. **Indicates significant difference between treatments. EG = ethylene glycol; DMSO = dimethylsulphoxide. n = number of evaluated cells

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Porcine Health Management

ISSN: 2055-5660

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