Cough associated with the detection of Mycoplasma hyopneumoniae DNA in clinical and environmental specimens under controlled conditions

Table 2 Mycoplasma hyopneumoniae (MHP) DNA detection by specimen and room

Specimen	MHP DNA detection	Room
		1	2	3	4
		1 MHP-inoculated pig: 8 uninoculated pigs	3 MHP-inoculated pigs: 6 uninoculated pigs	6 MHP-inoculated pigs: 3 uninoculated pigs	9 MHP-inoculated pigs
Tracheal swab	First detection	DPI 3	DPI 3	DPI 3	DPI 3
	Pig-to-pig transmission detected^a	DPI 21	DPI 10	DPI 7	NA^b
	100% detection in group	DPI 52	DPI 38	DPI 21	DPI 7
	MHP PCR positive of total samples	47 of 99	69 of 107	94 of 104	98 of 103
	MHP PCR overall mean ± se Ct^c	26.8 ± 0.7	27.6 ± 0.6	26.2 ± 0.4	26.2 ± 0.4
	Highest MHP DNA concentration (DPI)	23.4 (DPI 59)	24.4 (DPI 45)	23.6 (DPI 28)	24.3 (DPI 28)
Oral fluid	First detection	DPI 8	DPI 9	DPI 14	DPI 8
	MHP PCR positive of total samples^d	17 of 59¹	32 of 59²	43 of 59²	31 of 59²
	MHP PCR overall mean ± se Ct	34.7 ± 0.3	33.3 ± 0.5	33.5 ± 0.3	33.4 ± 0.4
	MHP PCR lowest Ct	31.6 (DPI 51)	25.7 (DPI 13)	27.4 (DPI 45)	26.1 (DPI 28)
Drinker	First detection	DPI 21	DPI 21	DPI 21	DPI 21
	MHP PCR positive of total samples	5 of 8	4 of 8	4 of 8	5 of 8
	MHP PCR overall mean ± se Ct	35.3 ± 1.5	33.7 ± 1.9	34.7 ± 1.4	34.7 ± 1.6
	MHP PCR lowest Ct	34.5 (DPI 59)	31.5 (DPI 21)	33.3 (DPI 35)	32.2 (DPI 24)
Air	First detection	ND^e	DPI 10	ND^e	DPI 17
	MHP PCR positive of total samples	ND^e	5 of 26	ND^e	3 of 26
	MHP PCR overall mean ± se Ct		36.2 ± 0.4		36.3 ± 0.6

^aFirst pig-to-pig transmission based on detection of MHP DNA in deep tracheal swab from commingled animal
^bNA: not applicable
^cNegative values (Ct ≥ 37) were excluded from calculations of statistical descriptive measures
^dDifferent superscripted numbers (1, 2, 3, and 4) indicate statistical differences (P ≤ 0.05, logistic regression and Tukey–Kramer pairwise comparisons)
^eND: not done

ISSN: 2055-5660