Fig. 2

Principal component analysis for: A dataset-A, and B dataset-C. A Variance of serotonin receptors¹, and 12 immune parameters² coded as jejunum (J), colon (C) or peripheral blood mononuclear cells (PBMC), 7 immune cell types³, jejunum villus height and crypt depth, and weaning body weight (BW0). B Dataset-C—variance serotonin receptors¹, and immune parameters² (observation together regardless of tissue jejunum and colon). Data combines piglets at day 1 post-weaning (n = 9) and piglets at 15 days post-weaning (n = 10). ¹ mRNA expression (log 2) of serotonin (5-HT) receptors (5-HTR3, 5-HTR4, and 5-HTR7) for jejunum and colon. ²mRNA expression (log 2) of 11 cytokines: interleukin IL-1α (IL1a), IL-1β (IL1b), IL-6 (IL6), IL-8 (IL8), IL-10 (IL10), IL-12p35 (IL12a), IL-12p40 (IL12b), tumor necrosis factor alpha (TNFa), interferon alpha and gamma (IFNa and IFNg), transforming growth factor betta (TGFb) for jejunum, colon and PBMCs; and IgA-producing cells (IgAcell) for jejunum and colon. ³Lymphocytes B phenotype CD21+ (B), natural killer cells phenotype CD3−16+56+ (NK) and lymphocytes T phenotypes CD3+ (CD3), T helper CD3+CD4+ (Th), T cytotoxic CD3+CD8+ (Tc), CD4+CD8+ (CD4CD8), and CD4−CD8− (CD4CD8neg)