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Table 2 Primers for serotonin (5-HT) receptors 3, 4, and 7 designed1, cytokines2 and housekeeper genes3

From: Serotonin receptors and their association with the immune system in the gastrointestinal tract of weaning piglets

 

Primer forward (5′ → 3′)

Primer reverse (5′ → 3′)

References

5-HTR3

5′-AACTACAAGCCCCTCCAGGT-3′

5′-CATGAAAACACTCCTGTCGAA-3′

This paper

5-HTR4

5′-CGTCTGGATTTATGGGGAGA-3′

5′-GAACAAGATGACCCCTCTGC-3′

This paper

5-HTR5

5′-GCCACTTCTTCTGCAACGTC-3′

5′-CAAGCACACCTTGTCGTCAT-3′

This paper

IL-1α

5′-GTGCTCAAAACGAAGACGAACC-3′

5′-CATATTGCCATGCTTTTCCCAGAA-3′

[50]

IL-1β

5′-AACGTGCAGTCTATGGAGT-3′

5′-GAACACCACTTCTCTCTTCA-3′

[51]

IL-6

5′-CTGGCAGAAAACAACCTGAACC-3′

5′-TGATTCTCATCAAGCAGGTCTCC-3′

[51]

IL-8

5′-GCTCTCTGTGAGGCTGCAGTTC-3′

5′-AAGGTGTGGAATGCGTATTTATGC-3′

[52]

IL-10

5′-TGAGAACAGCTGCATCCACTTC-3′

5′-TCTGGTCCTTCGTTTGAAAGAAA-3′

[53]

IL-12p35

5′-AGTTCCAGGCCATGAATGCA-3′

5′-TGGCACAGTCTCACTGTTGA-3′

[53]

IL-12p40

5′-TTTCAGACCCGACGAACTCT-3′

5′-CATTGGGGTACCAGTCCAAC-3′

[54]

TNF-α

5′-ACTCGGAACCTCATGGACAG-3′

5′-AGGGGTGAGTCAGTGTGACC-3′

[55]

IFN-α

5′-CCCCTGTGCCTGGGAGAT-3′

5′-AGGTTTCTGGAGGAAGAGAAGGA-3′

[56]

IFN-γ

5′-TGGTAGCTCTGGGAAACTGAATG-3′

5′-GGCTTTGCGCTGGATCTG-3′

[53]

TGF-β

5′-CACGTGGAGCTATACCAGAA-3′

5′-TCCGGTGACATCAAAGGACA-3′

[56]

β-actin

5′-CTACGTCGCCCTGGACTTC-3′

5′-GATGCCGCAGGATTCCAT-3′

[57]

Cyclophilin

5′-TGCTTTCACAGAATAATTCCAGGATTTA-3′

5′-GACTTGCCACCAGTGCCATTA-3′

[58]

GAPDH

5′-ACATGGCCTCCAAGGAGTAAGA-3′

5′-GATCGAGTTGGGGCTGTGACT-3′

[58]

  1. 1Designed using Primer Blast® (Bethesda, MD, USA) from sequences XM_003357301.4, NM_001001267.1 and NM_214085.1
  2. 2Cytokines = interleukin (IL)-1α, IL1-β, IL-6, IL-8, IL-10, IL-12p35 (IL-12α), IL-12p40 (IL12-β), tumor necrosis factor alpha (TNF-α), interferon alpha and gamma (IFN-α and IFN-γ), transforming growth factor betta (TGF-β)
  3. 3β-actin, cyclophilin and glyceraldehyde-3-phosphatedehydrogenase (GAPDH)