Skip to main content

Table 2 Test characteristics for ELISA and PCR

From: Influence of parity and reproductive stage on the prevalence of Mycoplasma hyopneumoniae in breeding animals in belgian farrow-to-finish pig herds

 

PCR

Total

Positive

Negative

ELISA

Positive

127

508

635

Negative

13

152

165

 

Total

140

660

800

PPVELISA

20.0%

 

PPVPCR

90.7%

NPVELISA

92.1%

NPVPCR

23.0%

  1. On ten Belgian farrow-to-finish farms 80 (n = 800) tracheobronchial swabs and blood samples were collected from breeding animals. Swabs were tested for the presence of M. hyopneumoniae DNA with nested PCR and blood was analyzed for the presence of M. hyopneumoniae-specific antibodies with a commercial ELISA. The positive predictive value (PPV) and negative predictive value (NPV) were calculated for both tests. PCR was set as gold standard when the test characteristics for the ELISA were calculated, and vice versa