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Table 2 Test characteristics for ELISA and PCR

From: Influence of parity and reproductive stage on the prevalence of Mycoplasma hyopneumoniae in breeding animals in belgian farrow-to-finish pig herds

  PCR Total
Positive Negative
ELISA Positive 127 508 635
Negative 13 152 165
  Total 140 660 800
PPVELISA 20.0%   PPVPCR 90.7%
NPVELISA 92.1% NPVPCR 23.0%
  1. On ten Belgian farrow-to-finish farms 80 (n = 800) tracheobronchial swabs and blood samples were collected from breeding animals. Swabs were tested for the presence of M. hyopneumoniae DNA with nested PCR and blood was analyzed for the presence of M. hyopneumoniae-specific antibodies with a commercial ELISA. The positive predictive value (PPV) and negative predictive value (NPV) were calculated for both tests. PCR was set as gold standard when the test characteristics for the ELISA were calculated, and vice versa