| PCR | Total |
---|
Positive | Negative |
---|
ELISA | Positive | 127 | 508 | 635 |
Negative | 13 | 152 | 165 |
 | Total | 140 | 660 | 800 |
PPVELISA | 20.0% | Â | PPVPCR | 90.7% |
NPVELISA | 92.1% | NPVPCR | 23.0% |
- On ten Belgian farrow-to-finish farms 80 (n = 800) tracheobronchial swabs and blood samples were collected from breeding animals. Swabs were tested for the presence of M. hyopneumoniae DNA with nested PCR and blood was analyzed for the presence of M. hyopneumoniae-specific antibodies with a commercial ELISA. The positive predictive value (PPV) and negative predictive value (NPV) were calculated for both tests. PCR was set as gold standard when the test characteristics for the ELISA were calculated, and vice versa