Table 1 Daily schedule with sampling times

3:00–3:15 p.m. (previous day)

Application of treatment to all the mini-pigs

9:00–9:05 a.m

Collection of saliva from the first two mini-pigs (T₀)

9:05–9:15 a.m

Bringing of the two mini-pigs to the experimental room

9:15–9:20 a.m

5 min of calm for the pigs to acclimatise

9:20–9:30 a.m

Evaluation of skin lesions (before encounter)

9:30–9:45 a.m

Preparation of encounter, encounter, and end of encounter

9:45–9:50 a.m

Collection of saliva (T₁)

9:50–9:55 a.m

Evaluation of skin lesions (after encounter)

10:00–10:05 a.m

Collection of saliva (T₂)

10:15–10:20 a.m

Collection of saliva (T₃)

10:20–10:30 a.m

Bringing of the mini-pigs from the experimental room to the housing room

10:30–11:00 a.m

Cleaning procedure

11 a.m.-1:00 p.m

Re-start of the entire procedure with two other mini-pigs, from the beginning until the end