Table 3 Analyses of different pathogens, sample type and methods used on the piglets from both farms that were submitted to the laboratory
From: Premature farrowing and stillbirths in two organic sow farms due to riboflavin deficiency
Etiological agents | Number of samples taken | Sample type | Methods | References |
|---|---|---|---|---|
APPV | 9 | Pooled internal organs (brain, lungs, heart, kidney, spleen, lymph nodes) | RT-PCR | Schwarz et al. [14] |
EMCV | 13 | Heart | RT-PCR | Bakkali Kassimi et al. [13] |
PCV2 | 69 | Heart | qPCR real time | Olvera et al. [11] |
PCV3 | 32 | Heart | PCR real time | Palinski et al. [16] |
PCMV | 7 | Pooled internal organs (spleen, tonsils) | PCR | artus® CMV RG PCR Kit Qiagen |
PPV | 60 | Liver | PCR | Kim et al. [12] |
PRRSV | 120 | Spleen, lungs | RT-PCR real time | Virotype PRRS RT-PCR Kit (Kit Indical) |
PSV | 5 | Pooled internal organs (liver, spleen, lungs, intestines) | RT-PCR | La Rosa et al. [15] |
Brucella spp. | 25 | Pooled internal organs (spleen, uterus, testicles) | PCR real time | Bogdanovich et al. [19] |
Chlamydia spp. | 25 | Pooled internal organs (spleen, liver, lungs) | PCR real time | Ehricht et al. [17] |
Leptospira spp. | 25 | Pooled internal organs (lungs, liver, kidney) | PCR real time | Bedir et al. [18] |
Toxoplasma gondii | 24 | Brain, pooled internal organs (lungs, heart, liver, kidney, spleen, brain) | PCR real time | Menotti et al. [20] |