Fig. 5
From: Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model
A CLDN4, B OCLN, and C ZO-1 gene expression in IPEC-J2 monoculture upon LPS stimulation. Each value represents the mean ± SD of 8 replicates of 6 independent experiments. Asterisks indicate significant differences between groups (p < 0.05). Data were analyzed using the 2−ΔΔCt method, in which the expression levels of the gene, normalized to the expression of the reference gene HPRT1, were expressed as relative quantities (RQ). The analysis was performed by defining IPEC-J2 as reference group