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Fig. 8 | Porcine Health Management

Fig. 8

From: Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model

Fig. 8

A CLDN4, B OCLN, and C ZO-1 proteins in IPEC-J2 co-culture with PBMC with/without LPS and/or acetate or propionate treatment. Each value represents the mean ± SD of 8 replicates of 6 independent experiments. Letters indicate significant differences among groups (p < 0.05). Significant differences (p < 0.05) between each LPS-untreated group and the corresponding LPS-treated group are indicated with a hashtag (#). Data were normalized to the synthesis of the reference protein β-actin as relative intensity. The analysis was performed by defining untreated IPEC-J2 + PBMC as reference group (first histogram of each graph). The samples derived from the same experiment and the gels/blots were processed in parallel. The original blots are presented in Additional file 1: Figures S8 A, B, C

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