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Fig. 9 | Porcine Health Management

Fig. 9

From: Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model

Fig. 9

NF-κB gene expression in PBMC co-cultured with IPEC-J2 with or without LPS and/or acetate or propionate treatment. Each value represents the mean ± SD of 8 replicates of 6 independent experiments. Significant differences (p < 0.05) between each LPS-untreated group and the corresponding LPS-treated group are indicated with a hashtag (#). Different letters indicate significant differences among groups (p < 0.05). Data were analyzed using the 2−ΔΔCt method, in which the expression levels of the gene, normalized to the expression of the reference gene HPRT1, were expressed as relative quantities (RQ). The analysis was performed by defining untreated IPEC-J2 + PBMC as reference group (first histogram of the graph)

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