Table 2 Boar sperm characteristics and bacterial contamination in semen extended in BTS without antibiotic (NO-ANTIBIOTIC) or with antibiotic (ANTIBIOTIC) (least square means ± SEM)
Variables | Group | p value | |
|---|---|---|---|
| Â | NO-ANTIBIOTIC | ANTIBIOTIC | Â |
Total sperm motility (%) | 65.6 ± 3.4 | 67.5 ± 3.4 | 0.023 |
- Curvilinear velocity (VCL, µm/sec) | 83.4 ± 3.5 | 84.2 ± 3.5 | 0.204 |
- Straight-line velocity (VSL, µm/sec) | 24.6 ± 1.3 | 24.8 ± 1.3 | 0.686 |
- Average path velocity (VAP, µm/sec) | 44.0 ± 2.2 | 43.7 ± 2.2 | 0.799 |
Sperm viability (%) | 71.7 ± 2.1 | 72.8 ± 2.1 | 0.031 |
Acrosome integrity (%) | 78.5 ± 1.8 | 79.6 ± 1.8 | 0.011 |
Sperm membrane integrity (%) | 57.2 ± 2.7 | 58.2 ± 2.7 | 0.132 |
Sperm mitochondrial activity (%) | 67.7 ± 2.8 | 68.5 ± 2.8 | 0.141 |
Total bacterial count (CFU/mL, log10) | 3.9 ± 0.1 | 1.9 ± 0.1 | < 0.001 |