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Table 4 Comparison of boar sperm characteristics and bacterial contamination between NO-ANTIBIOTIC and ANTIBIOTIC groups examined on each storage day, in low- and high-viability semen samples (least square means ± SEM).

From: Bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage with or without antibiotics in a tropical environment

Variables

Day

Group

p value

  

NO-ANTIBIOTIC

ANTIBIOTIC

 

Low-viability semen samples

    

Total sperm motility (%)

0

64.0 ± 4.6

65.0 ± 4.6

NS

1

58.4 ± 4.6

58.9 ± 4.6

NS

2

54.3 ± 4.6

54.2 ± 4.6

NS

3

49.1 ± 4.6

49.6 ± 4.6

NS

Sperm viability (%)

0

69.4 ± 2.4

70.7 ± 2.4

NS

1

66.7 ± 2.4

67.0 ± 2.4

NS

2

64.0 ± 2.4

63.1 ± 2.4

NS

3

60.8 ± 3.4

59.4 ± 2.4

NS

Acrosome integrity (%)

0

79.1 ± 2.9

79.6 ± 2.9

NS

1

77.1 ± 2.9

76.9 ± 2.9

NS

2

73.2 ± 2.9

73.6 ± 2.9

NS

3

71.6 ± 2.9

71.9 ± 2.9

NS

Sperm membrane integrity (%)

0

56.5 ± 3.4

56.5 ± 3.4

NS

1

50.1 ± 3.4

50.8 ± 3.4

NS

2

46.1 ± 3.4

45.7 ± 3.4

NS

3

41.4 ± 3.4

40.2 ± 3.4

NS

Sperm mitochondrial activity (%)

0

66.1 ± 3.2

65.6 ± 3.2

NS

1

62.1 ± 3.2

61.2 ± 3.2

NS

2

59.3 ± 3.2

56.5 ± 3.2

NS

3

53.3 ± 3.2

51.1 ± 3.2

NS

Total bacterial count (CFU/mL, log10)

0

3.1 ± 0.2a

2.4 ± 0.2b

***

1

3.5 ± 0.2a

2.4 ± 0.2b

***

2

4.6 ± 0.2a

2.2 ± 0.2b

***

3

5.6 ± 0.2a

2.2 ± 0.2b

***

High-viability semen samples

    

Total sperm motility (%)

0

81.6 ± 2.1

83.5 ± 2.1

NS

1

77.1 ± 2.1

79.6 ± 2.1

NS

2

73.7 ± 2.1

77.3 ± 2.1

*

3

68.6 ± 2.1

73.6 ± 2.1

**

Sperm viability (%)

0

84.8 ± 1.2

86.0 ± 1.2

NS

1

79.7 ± 1.2

81.7 ± 1.2

NS

2

76.0 ± 1.2

79.0 ± 1.2

*

3

74.1 ± 1.2

77.0 ± 1.2

*

Acrosome integrity (%)

0

86.3 ± 1.0

87.1 ± 1.0

NS

1

84.2 ± 1.0

85.0 ± 1.0

NS

2

80.7 ± 1.0

83.6 ± 1.0

**

3

78.4 ± 1.0

81.2 ± 1.0

**

Sperm membrane integrity (%)

0

73.4 ± 1.4

74.3 ± 1.4

NS

1

66.0 ± 1.4

68.2 ± 1.4

NS

2

62.3 ± 1.4

65.6 ± 1.4

*

3

60.6 ± 1.4

63.8 ± 1.4

*

Sperm mitochondrial activity (%)

0

79.2 ± 2.1

81.7 ± 2.1

NS

1

77.4 ± 2.1

80.2 ± 2.1

NS

2

73.0 ± 2.1

76.9 ± 2.1

**

3

71.0 ± 2.1

74.7 ± 2.1

*

Total bacterial count (CFU/mL, log10)

0

2.6 ± 0.2

1.7 ± 0.2

***

1

2.9 ± 0.2

1.5 ± 0.2

***

2

4.1 ± 0.2

1.7 ± 0.2

***

3

5.1 ± 0.2

1.5 ± 0.2

***