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Table 3 Semen handling factors affecting boar semen quality

From: Boar management and semen handling factors affect the quality of boar extended semen

Factor

Details

Effect of semen quality

Reference

Collection

Latex gloves.

Toxic for the sperm

[76]

 

Preputial liquid into the collection container

Increases bacteriospermia

[68]

 

Collection longer than 7 min,

Increases bacteriospermia

[68]

Dilution

Temperature

Final dilution at 22 °C vs 30 °C (2-step dilution)

No differences in sperm motility, morphology or acrosome integrity after 3 days storage

[14]

 

Final dilution at 20 °C vs 32 °C (2-step dilution)

No differences on membrane integrity or responsiveness to capacitating conditions

[82]

 

Final dilution at 21 °C vs 32 °C (2-step dilution)

Lower motility, increased membrane damage after 6 days storage

[80]

Dilution rate

0.5 × 109 sperm/80 ml vs 2.5 × 109/80 ml

Lower motility

[83]

Storage media

Short vs long

Lower motility after 4 days storage

[15]a

 

Magnetized extender

Improve membrane integrity

[88]

 

Antibiotics

Prevent bacterial overgrowth

[92]

Packaging

Bags vs tubes.

Need less time to reach 17 °C

[96]

 

Some plastic compounds

Toxic for sperm

[97]

Storage

Temperature

< 12 °C

Reduced sperm motility and vitality

[78]

Duration

> 4 days

Reduced motility and fertility

[15, 86]

Air contact

Air contact during storage

Increase in pH, reduced sperm motility

[101]

Turning doses

180° rotation/12 h or five 360° rotations/h vs. non-rotated tubes, using

Increase in pH, reduced sperm motility

[102]

  1. aEffect only seen in 1 of the 3 investigated long term extenders compared to 2 short term extenders