Table 4 Experimental design of the study
Treatment | N | Vaccination | Challenge | Necropsy PCV-2 | Necropsy Mhyo | |
|---|---|---|---|---|---|---|
Left Side of the Neck (volume and route) | Right Side of the Neck (volume and route) | |||||
NV-NC | 6 | PBS (2.5 mL IM) | – | PBS | PCV-2 lesion assessment | Mhyo lung lesion scoring |
NV-C/PCV-2 | 15 | PBS (2.5 mL IM) | – | PCV-2b | PCV-2 lesion assessment | – |
VS-C/PCV-2 | 15 | Circovac (0.5 mL IM) | Hyogen (2.0 mL IM) | PCV-2b | – | |
VC-C/PCV-2 | 15 | Circovac + Hyogen (2.5 mL IM RTM) | – | PCV-2b | – | |
NV-C/Mhyo | 15 | PBS (2.5 mL IM) | – | Mhyo | – | Mhyo Lung lesion scoring |
VS-C/Mhyo | 15 | Circovac (0.5 mL IM) | Hyogen (2.0 mL IM) | Mhyo | – | |
VC-C/Mhyo | 15 | Circovac + Hyogen (2.5 mL IM RTM) | – | Mhyo | – | |