Fig. 3From: Evaluation of classical swine fever E2 (CSF-E2) subunit vaccine efficacy in the prevention of virus transmission and impact of maternal derived antibody interference in field farm applicationsEvaluation of vaccine induced immune response on sows and the passive immunity of piglets. Sows were immunized with two doses of CSF-E2 vaccine at 3 and 5 weeks before parturition (group D, n = 25) or one dose of LPC vaccine before insemination (group E, n = 35). a The CSF-specific antibody level was detected using an IDEXX CSFV Ab test kit to monitor vaccine induced immune response. The gray dotted line indicates a positive antibody response (blocking percentage > 40%). b The saliva CSFV RNA was assessed for 1 month after vaccination using real-time PCR. Pearson’s chi-square test with Yate’s continuity correction showed a significant difference between the CSFV positive count of groups D and E (p < 0.001). c The piglet serum samples randomly selected from CSF-E2 vaccinated sows (group F, n = 20) and LPC vaccinated sows (group G, n = 20) were analyzed by using the IDEXX CSFV Ab test kit. The mean blocking percentage of each group was calculated and Welch’s two-sample t-test was performed to evaluate the statistical difference between groups. *** p < 0.001Back to article page