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Table 1 Mycoplasma suis quantification of blood, urine and saliva samples determined by qPCR during experimental infection in group B animals (subcutaneously infected animals)

From: Update on shedding and transmission routes of porcine haemotrophic mycoplasmas in naturally and experimentally infected pigs

Days post infection M. suis/mL Animal ID (group B animal)
ID 23 ID 31 ID 32 ID 71 ID 73 ID 74 ID 76
2 Blood Negative Negative Negative Negative Negative Negative Negative
Urine Negative Negative Negative Negative Negative Negative Negative
Saliva Negative Negative Negative Negative Negative Negative Negative
4 Blood 2.2 × 103 Negative Negative Negative Negative 2.0 × 105 6.0 × 104
Urine Negative Negative Negative Negative Negative negative Negative
Saliva Negative Negative Negative Negative Negative Negative Negative
6 Blood 4.6 × 108 1.1 × 104 4.2 × 107 3.6 × 105 1.9 × 104 6.6 × 104 1.1 × 109
Urine Negative Negative Negative Negative Negative Negative Negative
Saliva Negative Negative Negative negative Negative Negative Negative
8 Blood 3.6 × 109 9.2 × 106 9.6 × 109 3.0 × 109 3.0 × 107 1.8 × 107 8.6 × 109
Urine Negative Negative Negative Negative 5.5 × 104 Negative Negative
Saliva Negative 2.0 × 103 Negative 2.84 × 103 Negative Negative Negative
14 Blood   1.4 × 108   1.9 × 107 3.6 × 108 2.2 × 107  
Urine   Negative   Negative Negative Negative  
Saliva   Negative   5.26 × 103 Negative Negative  
17* Blood   n.d   n.d 2.4 × 109 n.d  
Urine   n.d   n.d Negative n.d  
Saliva   n.d   n.d Negative n.d  
20* Blood   n.d   n.d   1.3 × 107  
Urine   n.d   n.d   Negative  
Saliva   n.d   n.d   Negative  
21 Blood   1.7 × 109   2.0 × 108    
Urine   Negative   Negative    
Saliva   Negative   Negative    
28 Blood   5.4 × 107   4.7 × 106    
Urine   Negative   3.5 × 104    
Saliva   Negative   Negative    
30* Blood   7.5 × 108   n.d    
Urine   Negative   n.d    
Saliva   Negative   n.d    
35 blood   4.4 × 105   7.2 × 107    
Urine   Negative   Negative    
Saliva   Negative   Negative    
41* Blood   9.2 × 107   n.d    
Urine   Negative   n.d    
Saliva   Negative   n.d    
42 Blood     1.8 × 107    
Urine     Negative    
Saliva     Negative    
48 Blood     1.4 × 109    
Urine     2.5 × 104    
Saliva     Negative    
49* Blood     1.9 × 108    
Urine     Negative    
Saliva     Negative    
56 Blood     1.0 × 104    
Urine     Negative    
Saliva     Negative    
60* Blood     2.9 × 109    
Urine     Negative    
Saliva     Negative    
62 Blood     4.9 × 107    
Urine     Negative    
Saliva     Negative    
  1. Bold lines represent a positive detection of M. suis
  2. n.d. not determined
  3. *Additional sampling date due to exacerbation of clinical signs, only clinically affected animals were sampled at this study date
  4. Euthanisation