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Table 1 Mycoplasma suis quantification of blood, urine and saliva samples determined by qPCR during experimental infection in group B animals (subcutaneously infected animals)

From: Update on shedding and transmission routes of porcine haemotrophic mycoplasmas in naturally and experimentally infected pigs

Days post infection

M. suis/mL

Animal ID (group B animal)

ID 23

ID 31

ID 32

ID 71

ID 73

ID 74

ID 76

2

Blood

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Urine

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Saliva

Negative

Negative

Negative

Negative

Negative

Negative

Negative

4

Blood

2.2 × 103

Negative

Negative

Negative

Negative

2.0 × 105

6.0 × 104

Urine

Negative

Negative

Negative

Negative

Negative

negative

Negative

Saliva

Negative

Negative

Negative

Negative

Negative

Negative

Negative

6

Blood

4.6 × 108

1.1 × 104

4.2 × 107

3.6 × 105

1.9 × 104

6.6 × 104

1.1 × 109

Urine

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Saliva

Negative

Negative

Negative

negative

Negative

Negative

Negative

8

Blood

3.6 × 109†

9.2 × 106

9.6 × 109†

3.0 × 109

3.0 × 107

1.8 × 107

8.6 × 109†

Urine

Negative

Negative

Negative

Negative

5.5 × 104

Negative

Negative

Saliva

Negative

2.0 × 103

Negative

2.84 × 103

Negative

Negative

Negative

14

Blood

 

1.4 × 108

 

1.9 × 107

3.6 × 108

2.2 × 107

 

Urine

 

Negative

 

Negative

Negative

Negative

 

Saliva

 

Negative

 

5.26 × 103

Negative

Negative

 

17*

Blood

 

n.d

 

n.d

2.4 × 109†

n.d

 

Urine

 

n.d

 

n.d

Negative

n.d

 

Saliva

 

n.d

 

n.d

Negative

n.d

 

20*

Blood

 

n.d

 

n.d

 

1.3 × 107†

 

Urine

 

n.d

 

n.d

 

Negative

 

Saliva

 

n.d

 

n.d

 

Negative

 

21

Blood

 

1.7 × 109

 

2.0 × 108

   

Urine

 

Negative

 

Negative

   

Saliva

 

Negative

 

Negative

   

28

Blood

 

5.4 × 107

 

4.7 × 106

   

Urine

 

Negative

 

3.5 × 104

   

Saliva

 

Negative

 

Negative

   

30*

Blood

 

7.5 × 108

 

n.d

   

Urine

 

Negative

 

n.d

   

Saliva

 

Negative

 

n.d

   

35

blood

 

4.4 × 105

 

7.2 × 107

   

Urine

 

Negative

 

Negative

   

Saliva

 

Negative

 

Negative

   

41*

Blood

 

9.2 × 107†

 

n.d

   

Urine

 

Negative

 

n.d

   

Saliva

 

Negative

 

n.d

   

42

Blood

   

1.8 × 107

   

Urine

   

Negative

   

Saliva

   

Negative

   

48

Blood

   

1.4 × 109

   

Urine

   

2.5 × 104

   

Saliva

   

Negative

   

49*

Blood

   

1.9 × 108

   

Urine

   

Negative

   

Saliva

   

Negative

   

56

Blood

   

1.0 × 104

   

Urine

   

Negative

   

Saliva

   

Negative

   

60*

Blood

   

2.9 × 109

   

Urine

   

Negative

   

Saliva

   

Negative

   

62

Blood

   

4.9 × 107†

   

Urine

   

Negative

   

Saliva

   

Negative

   
  1. Bold lines represent a positive detection of M. suis
  2. n.d. not determined
  3. *Additional sampling date due to exacerbation of clinical signs, only clinically affected animals were sampled at this study date
  4. †Euthanisation
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Porcine Health Management

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