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Fig. 3 | Porcine Health Management

Fig. 3

From: DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development

Fig. 3

Cumulus cells genotoxicity assessment by the comet assay expressed by the Comet Tail Length (CTL) and DNA Integrity. Hydrogen peroxide (COCs exposed to 2.2% H2O2), Toxicity (16% EG + DMSO), and Vitrification (EG + DMSO + Vitrification). Cumulus cells (CCs) were removed from oocytes for evaluation. Control (n = 242 evaluated CCs), H2O2 (n = 263 evaluated CCs), Toxicity (n = 204 evaluated CCs), Vitrification (n = 219 evaluated CCs). The CTL refers to the extent of DNA damage, and DNA integrity to the percentage of DNA in the comet’s head (no DNA damage). Approximately < 15 μm of CTL is related to normal or undamaged DNA, and damaged > 30 μm. Higher CTL and lower DNA integrity was obtained in all groups compared to control. Data are presented as mean ± standard deviation (SD). Significant differences were considered when P < 0.05. *Indicates significant difference versus control. CTL = comet tail length; H2O2 = hydrogen peroxide; EG = ethylene glycol; DMSO = dimethylsulphoxide

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Porcine Health Management

ISSN: 2055-5660

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