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Fig. 5 | Porcine Health Management

Fig. 5

From: DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development

Fig. 5

Cumulus cells comet assay evaluation. Representative images of comet assay evaluation. The direction of electrophoresis was left to right, and DNA fragments are observed as a comet tail. a Cumulus cells control magnification at 200×; no DNA migration. Scale bar: 15 μm. a’ Cumulus cells control: one cell magnification at 400×; no DNA migration. Scale bar: 15 μm. b Cumulus cells exposed to H2O2 magnification at 400×; DNA migration. Scale bar: 15 μm. c Cumulus cells exposed to EG + DMSO magnification at 400×; DNA migration. Scale bar: 15 μm. d Cumulus cells EG + DMSO + Vitrification group magnification at 400×; DNA migration. Dotted line indicates the CTL and the arrowhead the nucleoid of the cumulus cell. The percentage of DNA integrity is presented as less DNA damage, shown in a and a’ pictures

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Porcine Health Management

ISSN: 2055-5660

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