Table 9 Primers of the cytokines IFN-α, IFN-γ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p35 (IL-12α), IL-12p40 (IL-12β), TNF-α and TGF-β and primers of glyceraldehyde-3-phosphatedehydrogenase (GAPDH), cyclophilin and β-actin
|  | Primer forward (5′ → 3′) | Primer reverse (5′ → 3′) | References |
|---|---|---|---|
IFN-α | 5′-CCCCTGTGCCTGGGAGAT-3′ | 5′-AGGTTTCTGGAGGAAGAGAAGGA-3′ | [75] |
IFN-γ | 5′-TGGTAGCTCTGGGAAACTGAATG-3′ | 5′-GGCTTTGCGCTGGATCTG-3′ | [76] |
IL-1α | 5′-GTGCTCAAAACGAAGACGAACC-3′ | 5′-CATATTGCCATGCTTTTCCCAGAA-3′ | [77] |
IL-1β | 5′-AACGTGCAGTCTATGGAGT-3′ | 5′-GAACACCACTTCTCTCTTCA-3′ | [78] |
IL-6 | 5′-CTGGCAGAAAACAACCTGAACC-3′ | 5′-TGATTCTCATCAAGCAGGTCTCC-3′ | [78] |
IL-8 | 5′-GCTCTCTGTGAGGCTGCAGTTC-3′ | 5′-AAGGTGTGGAATGCGTATTTATGC-3′ | [79] |
IL-10 | 5′-TGAGAACAGCTGCATCCACTTC-3′ | 5′-TCTGGTCCTTCGTTTGAAAGAAA-3′ | [77] |
IL-12p35 | 5′-AGTTCCAGGCCATGAATGCA-3′ | 5′-TGGCACAGTCTCACTGTTGA-3′ | [75] |
IL-12p40 | 5′-TTTCAGACCCGACGAACTCT-3′ | 5′-CATTGGGGTACCAGTCCAAC-3′ | [80] |
TNF-α | 5′-ACTCGGAACCTCATGGACAG-3′ | 5′-AGGGGTGAGTCAGTGTGACC-3′ | [81] |
TGF-β | 5′-CACGTGGAGCTATACCAGAA-3′ | 5′-TCCGGTGACATCAAAGGACA-3′ | [76] |
β-actin | 5′-CTACGTCGCCCTGGACTTC-3′ | 5′-GATGCCGCAGGATTCCAT-3′ | [82] |
Cyclophilin | 5′-TGCTTTCACAGAATAATTCCAGGATTTA-3′ | 5′-GACTTGCCACCAGTGCCATTA-3′ | [83] |
GAPDH | 5′-ACATGGCCTCCAAGGAGTAAGA-3′ | 5′-GATCGAGTTGGGGCTGTGACT-3′ | [83] |