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Table 9 Primers of the cytokines IFN-α, IFN-γ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p35 (IL-12α), IL-12p40 (IL-12β), TNF-α and TGF-β and primers of glyceraldehyde-3-phosphatedehydrogenase (GAPDH), cyclophilin and β-actin

From: Fermented rye with Agaricus subrufescens and mannan-rich hydrolysate based feed additive to modulate post-weaning piglet immune response

  Primer forward (5′ → 3′) Primer reverse (5′ → 3′) References
IFN-α 5′-CCCCTGTGCCTGGGAGAT-3′ 5′-AGGTTTCTGGAGGAAGAGAAGGA-3′ [75]
IFN-γ 5′-TGGTAGCTCTGGGAAACTGAATG-3′ 5′-GGCTTTGCGCTGGATCTG-3′ [76]
IL-1α 5′-GTGCTCAAAACGAAGACGAACC-3′ 5′-CATATTGCCATGCTTTTCCCAGAA-3′ [77]
IL-1β 5′-AACGTGCAGTCTATGGAGT-3′ 5′-GAACACCACTTCTCTCTTCA-3′ [78]
IL-6 5′-CTGGCAGAAAACAACCTGAACC-3′ 5′-TGATTCTCATCAAGCAGGTCTCC-3′ [78]
IL-8 5′-GCTCTCTGTGAGGCTGCAGTTC-3′ 5′-AAGGTGTGGAATGCGTATTTATGC-3′ [79]
IL-10 5′-TGAGAACAGCTGCATCCACTTC-3′ 5′-TCTGGTCCTTCGTTTGAAAGAAA-3′ [77]
IL-12p35 5′-AGTTCCAGGCCATGAATGCA-3′ 5′-TGGCACAGTCTCACTGTTGA-3′ [75]
IL-12p40 5′-TTTCAGACCCGACGAACTCT-3′ 5′-CATTGGGGTACCAGTCCAAC-3′ [80]
TNF-α 5′-ACTCGGAACCTCATGGACAG-3′ 5′-AGGGGTGAGTCAGTGTGACC-3′ [81]
TGF-β 5′-CACGTGGAGCTATACCAGAA-3′ 5′-TCCGGTGACATCAAAGGACA-3′ [76]
β-actin 5′-CTACGTCGCCCTGGACTTC-3′ 5′-GATGCCGCAGGATTCCAT-3′ [82]
Cyclophilin 5′-TGCTTTCACAGAATAATTCCAGGATTTA-3′ 5′-GACTTGCCACCAGTGCCATTA-3′ [83]
GAPDH 5′-ACATGGCCTCCAAGGAGTAAGA-3′ 5′-GATCGAGTTGGGGCTGTGACT-3′ [83]