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Table 3 Cough indices and pathologic lesions associated with Mycoplasma hyopneumoniae (MHP) infection by room

From: Cough associated with the detection of Mycoplasma hyopneumoniae DNA in clinical and environmental specimens under controlled conditions

Clinical assessment and pathology

Rooma

1

2

3

4

5

Cough counted per pig over 27-min (blind observer to MHP status)b

     

 First observation

DPI 21

DPI 10

DPI 14

DPI 10

-

 Coughs per 27 min (mean ± se)

5.8 ± 1.9

8.7 ± 2.1

18.5 ± 4.1

18.3 ± 6.7

-

 Coughing pigs per day (mean ± se)

1.9 ± 0.8

1.8 ± 1.0

3.8 ± 2.0

4.6 ± 2.5

0

 Number of days with coughing pigs

9

13

14

9

0

 Total number of coughs

991

1471

3142

2922

0

 Maximum cough (number of coughs)

DPI 56 (26)

DPI 24 (30)

DPI 38 (50)

DPI 21 (79)

-

Coughs recorded over 24-h (SoundTalk®, SoundTalks NV, Leuven, Belgium)c

     

 Coughs per 24 h (mean ± se)

23.4 ± 4.1

55.6 ± 6.6

33.1 ± 4.1

76.6 ± 11.2

23.1 ± 3.6

 Total number of coughs

9591

2,2792,3

1,3901,2

3,2923

9921

 Maximum cough (number of coughs)

DPI 34 (139)

DPI 39 (152)

DPI 30 (108)

DPI 22 (232)

DPI 54 (83)

 Respiratory distress index (mean ± se)

0.02 ± 0.01

0.17 ± 0.03

0.04 ± 0.01

0.12 ± 0.02

0.01 ± 0.01

 Maximum respiratory distress index

DPI 34 (0.20)

DPI 31 (0.58)

DPI 30 (0.22)

DPI 35 (0.53)

DPI 3 (0.41)

Lung score

     

 Median gross lesion (min–max)

2.9% (0.6–36)

1.1% (0–12)

1.1% (0–18)

0.4% (0–3.8)

0% (0–0)

 Median microscopic score (min–max)

4 (3–4)

3 (1–4)

4 (1–4)

3 (2–4)

1 (1–1)

  1. aRoom 1: One MHP-inoculated pig commingled with 8 uninoculated pigs. Room 2: 3 MHP-inoculated pigs with 6 uninoculated pigs. Room 3: 6 MHP-inoculated pigs with 3 uninoculated pigs. Room 4: 9 MHP-inoculated pigs. Room 5: 3 uninoculated pigs
  2. bDifferent superscripted numbers (1 or 2) indicate statistical differences among Room groups (P ≤ 0.05, negative binomial regression and Tukey–Kramer pairwise comparisons)
  3. cDifferent superscripted numbers (1, 2, and 3) indicate statistical differences among Room groups (P ≤ 0.05, negative binomial regression and Tukey–Kramer pairwise comparisons)