Table 1 Target genes and details of the primer sequences used for quantitative SYBR® Green real-time PCR amplification. The HPRT-1 gene was used as endogenous control gene
Target gene | GenBank accession nr | Primer sequence | Efficiency (%) | Slope | r2 | Amplicon length (bp) |
|---|---|---|---|---|---|---|
BD-1 [44] | NM_213838 | F 5′-CAGCCACCAGCATGAGACT-3′ R 5′-CAGGTAACAGGACCATGAGGA-3′ | 101.47 | − 3.28 | 0.99 | 63 |
CLDN4 [44] | AB235916 | F 5’-TATCATCCTGGCCGTGCTA-3’ R 5’-CATCATCCACGCAGTTGGT-3’ | 100.2 | − 3.29 | 0.99 | 71 |
OCLN [45] | FN400888 | F 5’-GGAGTGATTCGGATTCTGTCTATGCT-3’ R 5’-CGCCTGGGCTGTTGGGTTGA-3’ | 103.8 | − 3.03 | 0.99 | 423 |
ZO-1 [45] | CV870309 | F 5′-GGCGCACGGCGAAGGTAATT-3′ R 5′-CTATCAAACTCAGGAGGCGGCACT-3’ | 103.7 | − 3.21 | 0.98 | 405 |
NF-κB [44] | DQ834921 | F 5’-GAAGGACCTCTAGAAGGCAAAA-3’ R 5’-GCTTTGGTTTATGCGGTGTT-3’ | 104.2 | − 3.22 | 0.99 | 63 |
TNF-α [46] | NM_214022 | F 5’-ACTGCACTTCGAGGTTATCGG-3’ R 5’-GGCGACGGGCTTATCTGA-3’ | 98.5 | − 3.36 | 0.99 | 118 |
HPRT-1 [44] | DQ845175 | F 5’-ACACTGGCAAAACAATGCAA-3’ R 5’-TGCAACCTTGACCATCTTTG-3’ | 102.01 | − 3.25 | 0.99 | 71 |