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Table 9 Effects of guanidinoacetic acid (GAA) on splenic free amino acid profile in finishing pigs (mg/kg dry spleen)

From: Guanidine acetic acid supplementation altered plasma and tissue free amino acid profiles in finishing pigs

Items GAA, mg/kg SEM P-value
0 500 1000 1500 ANOVA Linear Quadratic
EAA
Methionine 0.09 0.07 0.20 0.19 0.01 0.002 0.001 0.491
Threonine 3.28b 3.40b 5.17a 4.82a 0.16  < 0.001  < 0.001 0.159
Valine 4.70b 4.63b 7.31a 7.14a 0.18  < 0.001  < 0.001 0.695
Isoleucine 1.56b 1.57b 2.42a 2.32a 0.06  < 0.001  < 0.001 0.398
Leucine 12.16b 11.77b 18.51a 18.96a 0.54  < 0.001  < 0.001 0.538
Phenylalanine 4.48b 4.39b 6.82a 6.76a 0.19  < 0.001  < 0.001 0.965
Tryptophan 1.82b 1.83b 9.26a 9.10a 0.36  < 0.001  < 0.001 0.706
Lysine 5.24b 5.26b 7.82a 7.11a 0.29  < 0.001  < 0.001 0.208
Histidine 1.96c 1.90c 2.93a 2.57b 0.09  < 0.001  < 0.001 0.087
Arginine 4.14b 3.98b 6.29a 5.63a 0.21  < 0.001  < 0.001 0.287
NEAA
Serine 4.41b 4.58b 7.05a 6.77a 0.21  < 0.001  < 0.001 0.312
Glycine 14.67b 14.54b 20.31a 17.07ab 0.97 0.007 0.025 0.179
Alanine 6.29b 6.54b 9.04a 9.36a 0.35  < 0.001  < 0.001 0.987
Glutamate 35.20b 33.73b 44.92a 36.18b 1.83 0.004 0.148 0.079
Glutamine 0.48b 0.43b 1.06a 1.06a 0.05  < 0.001  < 0.001 0.700
Aspartic acid 10.45bc 9.40c 15.11a 12.54ab 0.73  < 0.001 0.002 0.270
Asparagine 1.90b 1.93b 2.92a 2.56a 0.12  < 0.001  < 0.001 0.127
Cystine 0.19 0.24 0.23 0.23 0.04 0.895 0.691 0.597
Tyrosine 3.40b 3.39b 5.08a 5.06a 0.16  < 0.001  < 0.001 0.906
Proline 3.05b 2.71b 5.24a 5.20a 0.22  < 0.001  < 0.001 0.639
Non-protein amino acids and derivatives/metabolites of amino acids
Ornithine 0.08 0.09 0.08 0.13 0.04 0.901 0.530 0.774
Taurine 31.59 31.81 33.36 37.29 1.77 0.171 0.044 0.352
Citrulline 0.27 0.26 0.28 0.35 0.03 0.250 0.085 0.295
β-alanine 0.49 0.35 0.44 0.42 0.06 0.571 0.771 0.366
Cystathionine 5.10b 5.01b 7.55a 8.15a 0.25  < 0.001  < 0.001 0.245
α-aminoadipic acid 1.70b 1.80ab 2.05ab 2.24a 0.11 0.017 0.002 0.718
α-amino-n-butyric acid 0.81 0.81 1.12 0.84 0.14 0.403 0.592 0.351
β-aminoisobutyric acid 1.23b 1.23b 1.42ab 1.68a 0.09 0.012 0.002 0.199
Phosphoethanolamine 11.92ab 9.92b 17.43a 14.03ab 1.38 0.009 0.044 0.584
Urea 2.47 3.20 2.60 3.66 0.32 0.347 0.197 0.956
Ammonia 2.45 2.08 2.18 1.97 0.15 0.200 0.076 0.602
∑EAA 39.37b 38.74b 66.66a 64.60a 1.49  < 0.001  < 0.001 0.549
∑NEAA 121.50b 119.18b 156.95a 146.85a 3.61  < 0.001  < 0.001 0.230
TAA 160.87b 157.92b 223.61a 211.45a 3.93  < 0.001  < 0.001 0.256
  1. SEM standard error of the mean, EAA essential amino acids, NEAA nonessential amino acids, TAA total amino acids
  2. Dietary treatments were: basal diet + 0, 500, 1000, and 1500 mg/kg GAA, respectively. n = 6
  3. a, bMean within rows with different letters differ significantly (P < 0.05)
  4. Bold indicates P < 0.05