Table 1 Target genes and primer sequences used for SYBR Green qPCR
From: Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model
Target gene | GenBank Accession nr | Primer sequence | Efficiency (%) | Slope | r2 | Amplicon length (bp) |
|---|---|---|---|---|---|---|
CLDN4 [31] | AB235916 | F 5′-TATCATCCTGGCCGTGCTA-3′ R 5′-CATCATCCACGCAGTTGGT-3′ | 100.2 | − 3.29 | 0.99 | 71 |
OCLN [32] | FN400888 | F 5′-GGAGTGATTCGGATTCTGTCTATGCT-3′ R 5′-CGCCTGGGCTGTTGGGTTGA-3′ | 103.8 | − 3.03 | 0.99 | 423 |
ZO1 [32] | XM_021098896 | F 5′-GGCGCACGGCGAAGGTAATT-3′ R 5′-CTATCAAACTCAGGAGGCGGCACT-3′ | 103.7 | − 3.21 | 0.98 | 405 |
HPRT-1 [31] | DQ845175 | F 5′-ACACTGGCAAAACAATGCAA-3′ R 5′-TGCAACCTTGACCATCTTTG-3′ | 102.0 | − 3.25 | 0.99 | 71 |
GAPDH [15] | NM_001206359 | F 5′-GGTGAAGGTCGGAGTGAACG-3′ R 5′-GCCAGAGTTAAAAGCAGCCCT-3′ | 102.0 | − 3.27 | 0.99 | 70 |
β-2MG [33] | DQ17123 | F 5′-AAACGGAAAGCCAAATTACC-3′ R 5′-ATCCACAGCGTTAGGAGTGA-3′ | 106.3 | − 3.19 | 0.98 | 178 |
18S rRNA [34] | AY265350.1 | F 5′-CCCACGGAATCGAGAAAGAG-3′ R 5′-TTGACGGAAGGGCACCA-3′ | 100.9 | − 3.30 | 0.99 | 125 |
NF-κB [31] | DQ834921 | F 5′-GAAGGACCTCTAGAAGGCAAAA-3′ R 5′-GCTTTGGTTTATGCGGTGTT-3′ | 99.6 | − 3.21 | 0.99 | 125 |