Effects of dietary supplementation with a carvacrol–cinnamaldehyde–thymol blend on growth performance and intestinal health of nursery pigs

Table 5 The relative mRNA expression of intestinal epithelial integrity-related genes as affected by dietary CEO supplementation ¹

Item	Con	CEO	SEM	P-value
Duodenum
ZO-1	1.00	1.98	0.49	0.025
Claudin-1	1.00	1.93	0.47	0.035
Claudin-5	1.00	2.04	0.52	0.014
Occludin	1.00	2.07	0.53	0.012
E-cadherin	1.00	1.30	0.15	0.904
Jejunum
ZO-1	1.00	1.10	0.05	0.911
Claudin-1	1.00	1.13	0.07	0.797
Claudin-5	1.00	1.28	0.14	0.109
Occludin	1.00	1.93	0.46	< 0.001
E-cadherin	1.00	2.30	0.65	< 0.001
Ileum
ZO-1	1.00	2.57	0.79	< 0.001
Claudin-1	1.00	3.22	1.11	< 0.001
Claudin-5	1.00	2.00	0.50	< 0.001
Occludin	1.00	1.33	0.17	0.366
E-cadherin	1.00	1.88	0.44	< 0.001

¹ The data were analyzed following the 2^−ΔΔCt method, and then the target gene transcript levels were normalized to β-actin

ISSN: 2055-5660