Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain

Table 3 Primer sets used for the detection of porcine enteric viruses by multiplex RT-PCR

Virus	Primer	Sequence (5'–3')	Target	Size (bp)	References
RT-PCR multiplex 1
TGEV	TGEV-TA	GATGGCGACCAGATAGAAGT	Nucleocapsid gene	612	[83]
	TGEV-TB	GCAATAGGGTTGCTTGTACC
PToV	ToV-F	ATTGCTTATTGGTGGCTTCC	Spike gene	464	This study
	ToV-R	GGCTACTCAAACTTAACACT
PAstV	AstV-F	ATGTCTTTGGGATGTGG	RdRp gene	334	This study
	AstV-R	TTTGGTCCTCCCCTCCAAAG
RT-PCR multiplex 2
PEDV	PEDV-F	TTCTGAGTCACGAACAGCCA	Spike gene	651	[84]
	PEDV-R	CATATGCAGCCTGCTCTGAA
PKoV	KV-F1	CGGGACTGGTTTGGAGGAACA	Leader gene	446	[24]
	KV-R	CTCTATCAAGCAGTACATGG			This study
RT-PCR multiplex 3
RVC	RVC-VP7-F	GCTGTCTGACAAACTGGTC	Viral protein 7 gene	1061	[85]
	RVC-VP7-R	GCCACATGATCTTGTTTACGC
MRV	MRV-L1-F	CTCGTGAAATGGCGAATGTG	RdRp Lambda 3 gene	638	This study
	MRV-L1-R	TAGACTCACGCTGACCGTCC
RT-PCR multiplex 4
RVA	RVA-NSP2-F	GGCTTTTAAAGCGTCTCAGTC	Non-structural protein 2 gene	1059	[86]
	RVA-NSP2-R	GGTCACATAAGCGCTTTCTATTC
PAdV	AdV-PALF	GATGTCATGGAYAACGTCAAC	Hexon gene	612	[36]
	AdV-PARF	CACGGAGGAGTCRAACTGGATG

MRV: Mammalian orthoreovirus, PAdV: Porcine mastadenovirus, PAstV: Porcine astrovirus, PEDV: Porcine epidemic diarrhoea virus, PKoV: Porcine kobuvirus, PToV: Porcine torovirus, RVA: Rotavirus A, RVC: Rotavirus C and TGEV: Transmissible gastroenteritis virus

ISSN: 2055-5660