Fig. 2

Effect of SPCI on ZO-1 distribution and apoptosis of the intestinal epithelium cells

(A) Localization of ZO-1 and DAPI (DNA) in the jejunum. ZO-1 protein (green), DAPI stain (blue) as well as merged ZO-1 protein and DAPI are presented; (B) The mean gray value of ZO-1; (C) Evaluation of jejunal cell apoptosis by flow cytometry in weaned pigs. 30,000 cells were used in each acquisition reading. Frames were divided into 4 quadrants: Q1-UL represents necrotic cells; Q1-UR represents late apoptotic and early necrotic cells; Q1-LR represents early apoptotic cells; and Q1-LL represents normal cells; Percentages of apoptotic cells of early apoptosis (D), late apoptosis (E), total apoptosis (F) in the jejunum, respectively. a,b,c Mean values with different letters on vertical bars indicate significant differences (P < 0·05). T1, control (basal diet); T2, the basal diet containing 50 mg/kg SPCI ; T3, the basal diet containing 75 mg/kg SPCI ; T4, the basal diet containing 100 mg/kg SPCI; T5, the basal diet containing 125 mg/kg SPCI