Table 1 List of methods used to analyse the biomarkers included in the trial
From: Analysing biomarkers in oral fluid from pigs: influence of collection strategy and age of the pig
Biomarker | Method | Reference |
|---|---|---|
Stress | ||
Cortisol | In house AlphaLISA assay using a commercial monoclonal antibody | Lopez-Arjona et al., 2020 [19] |
Amylase | Commercial spectrophotometric assay | Fuentes et al., 2011 [20] |
Oxytocin | In house AlphaLISA assay using an in house monoclonal antibody | Lopez-Arjona et al., 2020 [19] |
Inflammation | ||
Haptoglobin | In house AlphaLISA assay using an in house monoclonal antibody | Contreras-Aguilar et al., 2021 [21] |
Sepsis | ||
Procalcitonin | In house AlphaLISA assay using an in house polyclonal antibody | López-Martínez et al. 2022 [22] |
Immune system | ||
ADA | In house spectrophotometric assay | Tecles et al. 2018 [13] |
Immunoglobulin G | Commercial sandwich Elisa | Navarro et al. 2021 [23] |
Oxidative stress | ||
FRAP | In house spectrophotometric assay | Rubio et al. 2019 [14] |
General homeostasis | ||
Protein | Commercial colorimetric kit | Ortín-Bustillo et al. 2022 [7] |
Creatine Kinase | Commercial colorimetric kit | Ortín-Bustillo et al. 2022 [7] |
LDH | Commercial colorimetric kit | Ortín-Bustillo et al. 2022 [7] |