Fig. 3

Molecular characteristics of NSP2 and GP5 of L1.8(L1C) PRRSV. a NSP2 deletion characteristics of L1.8(L1C) PRRSV on pig farms. The positions marked in the figure represent positions of the NSP2 amino acid sequence and refer to the position of ATCC VR2332. Purple indicates the NADC30-like PRRSV 131 aa characteristic discontinuous deletion. Additional aa deletions are indicated in sky blue, and their proportions are indicated on the right side of the sequence. b Alignment of full-length GP5 aa sequences of part-positive L1.8(L1C) PRRSV strains. Upper grey rectangles show antigenic regions (PNE—principal neutralizing epitope), and lower grey rectangles show biologically significant regions (Signal sequence; TM—transmembrane region). The rightmost digit of the aa sequence is the proportion of GP5 sequences with L1.8(L1C) molecular characteristics identical on a single farm. The aa mutation of L1.8(L1C) on a given pig farm are coloured differently than the reference strain. Positive selection sites are marked with red fivepointed stars