Table 1 The primer used in this study
Virus | Primer | Sequence | Position | Product Size | Target gene |
|---|---|---|---|---|---|
PEDV | PEDV-F | 5’-GGTTGCTACTGGCGTACAGGTA-3’ | 26,148–26,169 | 105 bp | M gene |
PEDV-R | 5’-GAAGCATTGACTGAACGACCAACA-3’ | 26,229–26,252 | |||
PEDV-P | 5’-FAM-TCGTCACAGTCGCCAAGGCCACTACAACA-BHQ1-3’ | 26,186–26,213 | |||
TGEV | TGEV-F | 5’-GCGTTAGTGCATTAGGAAGAAGCTA-3’ | 26,562–26,586 | 91 bp | M gene |
TGEV-R | 5’-GCGTACAAATTCCCTGAAAGCAAAG-3’ | 26,628–26,652 | |||
TGEV-P | 5’-HEX-CCTCTCGAAGGTGTGCCAACTGGTGTCACTCT-BHQ1-3’ | 26,594–26,625 | |||
RVA | RVA-F | 5’-TGAATCGTCTTCTACAACGTCAAC-3’ | 81–104 | 147 bp | NSP5 |
RVA-R | 5’-TCGTTTGAAGCAGAATCAGATGG-3’ | 205–227 | |||
RVA-P | 5’-ROX-CTCTGGAGACTTCGACAACAT-MGB-3’ | 175–195 | |||
PDCoV | PDCoV-F | 5’-GGTCGTTAACCAGACCTATGAG-3’ | 24,847–24,868 | 177 bp | N gene |
PDCoV-R | 5’-GCTGCTGATTCCTGCTTTATCTC-3’ | 25,001–25,023 | |||
PDCoV-P | 5’-CY5-CCAACTAAGGACAAGAAGCCTGACA-BHQ3-3’ | 24,881–24,905 | |||
PEDV | PEDV-F1 | 5’-GGCGGTTCTTTTCAAAATTTAAT-3’ | 20,731–20,753 | 1981 bp | Partial S1 gene |
|  | PEDV-R1 | 5’-GCACCACTAGTGACATTCTTAAA-3’ | 22,689–22,711 | ||
RVA | RVA-F1 | 5’-GGCTTTAAAAGAGAGAATTTC-3’ | 1–21 | 1060 bp | VP7 gene |
RVA-R1 | 5’-GGTCACATCATACAATTC-3’ | 1043–1060 |