Table 2 Sensitivity and repeatability of multiplex qRT-PCR
Plasmid | Copy number | Ct(mean ± S.D.) | CV% | Plasmid | Copy number | Ct(mean ± S.D.) | CV% | Plasmid | Copy number | Ct(mean ± S.D.) | CV% | Plasmid | Copy number | Ct(mean ± S.D.) | CV% |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PEDV | 5.0 × 109 | 8.09 ± 0.02 | 0.23% | TGEV | 5.0 × 109 | 8.16 ± 0.03 | 0.42% | RV | 5.0 × 109 | 8.30 ± 0.06 | 0.74% | PDCoV | 5.0 × 109 | 8.03 ± 0.09 | 1.09% |
5.0 × 108 | 11.85 ± 0.05 | 0.45% | 5.0 × 108 | 11.79 ± 0.03 | 0.28% | 5.0 × 108 | 11.85 ± 0.05 | 0.45% | 5.0 × 108 | 11.52 ± 0.07 | 0.60% | ||||
5.0 × 107 | 15.38 ± 0.04 | 0.27% | 5.0 × 107 | 15.36 ± 0.05 | 0.32% | 5.0 × 107 | 15.42 ± 0.03 | 0.22% | 5.0 × 107 | 14.87 ± 0.06 | 0.43% | ||||
5.0 × 106 | 19.19 ± 0.04 | 0.22% | 5.0 × 106 | 18.92 ± 0.02 | 0.13% | 5.0 × 106 | 19.11 ± 0.05 | 0.26% | 5.0 × 106 | 18.35 ± 0.07 | 0.38% | ||||
5.0 × 105 | 22.74 ± 0.05 | 0.22% | 5.0 × 105 | 22.46 ± 0.05 | 0.24% | 5.0 × 105 | 22.61 ± 0.06 | 0.27% | 5.0 × 105 | 21.83 ± 0.10 | 0.34% | ||||
5.0 × 104 | 26.36 ± 0.06 | 0.22% | 5.0 × 104 | 26.1 ± 0.04 | 0.17% | 5.0 × 104 | 26.21 ± 0.07 | 0.29% | 5.0 × 104 | 25.31 ± 0.06 | 0.24% | ||||
5.0 × 103 | 29.85 ± 0.07 | 0.23% | 5.0 × 103 | 29.57 ± 0.06 | 0.19% | 5.0 × 103 | 29.85 ± 0.08 | 0.26% | 5.0 × 103 | 28.72 ± 0.08 | 0.25% | ||||
5.0 × 102 | 33.51 ± 0.08 | 0.23% | 5.0 × 102 | 33.25 ± 0.07 | 0.22% | 5.0 × 102 | 33.34 ± 0.07 | 0.22% | 5.0 × 102 | 32.25 ± 0.08 | 0.24% | ||||
5.0 × 101 | 36.03 ± 0.07 | 0.20% | 5.0 × 101 | 36.64 ± 0.10 | 0.26% | 5.0 × 101 | 36.77 ± 0.09 | 0.24% | 5.0 × 101 | 35.64 ± 0.12 | 0.20% | ||||
10 | 36.70 ± 0.13 | 0.35% | 10 | 36.78 ± 0.14 | 0.39% | 10 | 37.10 ± 0.12 | 0.32% | 10 | 36.42 ± 0.17 | 0.46% | ||||
5 | 37.36 ± 0.19 | 0.50% | 5 | 37.41 ± 0.10 | 0.26% | 9 | 37.70 ± 0.15 | 0.39% | 9 | 37.08 ± 0.17 | 0.47% | ||||
4 | 37.30 ± 0.23 | 0.62% | 4 | 37.72 ± 0.12 | 0.32% | 8 | 37.70 ± 0.14 | 0.36% | 8 | 37.61 ± 0.13 | 0.35% | ||||
3 | 37.52 ± 0.26 | 0.69% | 3 | None | None | 5 | None | None | 5 | None | None | ||||
1 | None | None | 1 | None | None | 1 | None | None | 1 | None | None |